Skip to Content

Your Location Within Site:


Isolation and characterisation of a partially methylated galacto-glucurono-xylo-glycan, a unique polysaccharide from the red seaweed Apophloea lyallii

TitleIsolation and characterisation of a partially methylated galacto-glucurono-xylo-glycan, a unique polysaccharide from the red seaweed Apophloea lyallii
Publication TypeJournal Article
Year of Publication2002
AuthorsWatt, D. K., O'Neill S. A., Percy A. E., and Brasch D. J.
IRL TeamCarbohydrate Chemistry
JournalCarbohydrate Polymers
Volume50
Pagination283-294
Keywordschromatography, Ion exchange, ion exchange chromatography, nuclear magnetic resonance spectroscopy, Organic acids, Polysaccharides, seaweed
Abstract Polysaccharides of the red seaweed Apophloea lyallii were extracted with hot water in a yield of 63.6% w/w of the dry seaweed. The polysaccharides were non-sulphated and were composed of various proportions of D-xylose, 2-O-methyl D-galactose, D-glucuronic acid, D- and L-galactose, 3-O-methyl galactose, and D-glucose. The aldobiuronic acid 3-O-(β-D-glucopyranosyluronic acid)-D-xylopyranose was isolated from the acid hydrolysate of the polysaccharides. The polysaccharides were fractionated by DEAE-sephadex ion exchange chromatography, and two of the fractions obtained were characterised by neutral sugar analyses, linkage analyses, and NMR[?] spectroscopy. A major fraction obtained was shown to be largely composed of a unique (2-O-methyl galacto)-glucurono-xylo-glycan with a repeating unit represented by [α-D-(2-O-Me-Galp)-(1 → 4)-β-D-GlcAp-(1 → 3)-α-D-Xylp-(1 → 4)-]n. The structure of this new glycan was explored by beta-elimination analyses. A minor fraction obtained by ion exchange chromatography contained (1 → 4)-linked xylose, (1 → 4)-linked galactose, (1 → 4)-linked 3-O-methyl galactose, (1,3,4)-linked galactose, and terminal uronic acid in a ratio of approximately 1:0.3:0.5:0.4:0.4. © 2002 Elsevier Science Ltd. All rights reserved.
URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0037112940&partnerID=40&md5=31a9a9b4736106a267db9f06f1da4707
DOI10.1016/S0144-8617(02)00032-2

feedback